Fungal Stains For Diagnostic Mycology- Part 4

Fungal staining is the exquisite combination of stains that helps physicians to identify infections early. Since microbiological cultures might take over a month to produce a definitive diagnosis, this basic technique in clinical microscopy is boon for diagnostic mycology.

Fungal staining uses specific dyes that bind to the components of the fungal cell wall. Because of this binding, the fungi can be seen under a microscope, revealing their shape and structure. However, visibility is not the only factor. To help in the identification process, the way these dyes bond can also reveal a lot about the kind of fungus.

Medical practitioners can decide on the best course of action based on the type of fungus present in a sample. But which staining method, though, is the best for highlighting fungal features among the multitude of options? This question does not have a simple answer. Various staining methods reveal various things about the fungus. It is dependent upon the kind of fungus and the characteristics of the sample.

In certain circumstances, a mixture of staining methods may be applied. This can help identify the fungi by giving a more thorough view of them. However, understanding the benefits and drawbacks of each staining procedure is essential before choosing one. 

These methods are not merely instruments. They serve as evidence of the ability of science and technology to enhance medical results. They serve as a reminder that even microscopic organisms, such as fungus, have a big influence on human health and well being. Let's take a journey through the realm of fungal staining, investigating the specifics, as well as some obscure information and significant difficulties associated with this method, its use, and its importance.

Fungal stains part 4

References

1. Gridley MF. A stain for fungi in tissue sections. Am J Clin Pathol. 1953;23(3):303-7.

2. George A. Youngberg, Ellen D. B. Wallen, Tamar A. Giorgadze. Narrow-Spectrum Histochemical Staining of Fungi. Arch Pathol Lab Med 2003;127 (11): 1529–1530.

3. Shamly V, Kali A, Srirangaraj S, Umadevi S. Comparison of Microscopic Morphology of Fungi Using Lactophenol Cotton Blue (LPCB), Iodine Glycerol and Congo Red Formaldehyde Staining. J Clin Diagn Res. 2014;8(7):DL01-2.

4. Baddireddy K, Poojary S. A Novel Contrast Stain for the Rapid Diagnosis of Dermatophytoses: A Cross-Sectional Comparative Study of Chicago Sky Blue 6B Stain, Potassium Hydroxide Mount and Culture. Indian J Dermatol. 2019;64(4):311-314.

5. Heaton, S.M., Weintrob, A.C., Downing, K. et al. Histopathological techniques for the diagnosis of combat-related invasive fungal wound infections. BMC Clin Pathol 2016;16; 11.

6. Guarner J, Brandt ME. Histopathologic diagnosis of fungal infections in the 21st century. Clin Microbiol Rev. 2011;24(2):247-80.



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